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KMID : 0364820130490040419
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Korean Journal of Microbiology
2013 Volume.49 No. 4 p.419 ~ p.423
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Isolation and Characterization of Double Deletion Mutants of nsdD and veA or nsdC, the Genes Positive Regulating Sexual Development of Aspergillus nidulans
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Kim Lee-Han
Kim Ha-Eun
Han Dong-Min
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Abstract
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Three genes, nsdD, nsdC, and veA are known to be necessary for positive regulating sexual development of
Aspergillus nidulans. Since the mutants of those genes hardly form fruiting bodies in heterokaryons constructed by
cross between two of them, it is difficult to isolate double mutants. In this work, double mutants of ΔnsdD ΔveA and
ΔnsdD ΔnsdC were isolated using the characteristic of the nsdD deletion mutant that it could develop mature cleistothecia in hypoxic and low temperature culture condition. According to the phenotypes of double mutants, the nsdD gene controls the apical growth independently with veA or nsdC. Deletion of veA or nsdC was epistatic to nsdD deletion for pigment production. Conidia formation in submerged culture with lactose as sole carbon source was observed in ΔnsdD ΔnsdC double mutant implicating it to be unique phenotype of nsdC deletion.
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KEYWORD
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Aspergillus nidulans, nsdC, nsdD, veA, double mutants, sexual development
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